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Target gene expression levels were normalized to glyceraldehyde 3-phosphate dehydrogenase ( Gapdh ) expression using the 2 method

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The findings revealed that asthma-induced intestinal microbial imbalance led to an increase in short-chain fatty acids (SCFAs) production, a decrease in inflammatory factors such as IL-4 and IL-5, an elevation in the proportion of Treg cells, and a mitigation of airway inflammation in mice with allergic asthma induced by house dust mite (HDM)